Antibacterial, Antifungal and Antitubercular Activity of Methanolic Extracts of Adansonia digitata L

This study was aimed with an objective to investigate the antimicrobial, antifungal and antitubercular activity of different parts of Adansonia digitata L. The leaves, Root bark, and Fruit pulp were extracted with methanol. The antibacterial and antifungal activity of the extracts were determined using Broth dilution and Agar disc diffusion method and antitubercular activity was determined using L. J. agar (MIC) method. The antibacterial and antifungal activities of the extracts were tested against four Gram-positive strains Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumonia and Bacillus subtilis. Four Gramnegative strainsEscherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Vibrio cholera and three fungal strains Aspergillus niger, Aspergillus clavatus, Candida albicans. The results were compared with different standards like Ampicillin, Ciprofloxacin, Norfloxacin and Chloramphenicol for antibacterial activity and Nystatin and Griseofulvin for antifungal activity. The highest minimum inhibitory concentration (MIC) was showed by fruit pulp extract against E. coli and C. albicans at dose of 62μg/ml and 250μg/ml. Zone of inhibition studies revealed that the maximum inhibition was showed by fruit pulp extract. Antitubercular activity of the extracts was tested against bacteria Mycobacterium tuberculosis (H37Rv) and found that minimum inhibitory concentration was showed by root bark extract at dose 62.5μg/ml. The overall results of the minimal inhibitory concentration and zone of inhibition indicates that methanolic extracts of A. digitata L. contains some potent antibacterial, antifungal and antitubercular phytoconstituents.